View All Crystallisation at Instruct
The NKI Protein Facility has a strong record in sample preparation for structural biology, and offers their users access to macromolecular crystallisation:
- Robotics for preparation of nanoliter crystallisation drops in 96-well format.
- Storage of crystallisation plates (4°C or 20°C).
- Screening and ranking of crystallisation conditions in 96-well format (TOPAZ, AutoInspex station).
Solving experimental (crystal, crye-EM) structures of macromolecjules and their complexes is beyond the scope of the NKI Protein facility access services for Instruct-ERIC!
The Facility has access to a high-throughput macromolecular crystallisation platform. Liquid handling robots are available for preparation of nanoliter crystallisation drops in 96-well format, including a 96-well pipetting robot (CyBi-SELMA) for filling up reservoir wells and a Mosquito for creating nanoliter crystallisation drops. Crystallisation trials can be stored and imaged by two RockImager systems, one at 4 °C and one at 20 °C. When suitable crystals are obtained for x-ray diffraction analysis, the applicant has to collaborate with a crystallography facility. Solving crystal structures is beyond the scope of the facility.
Plesmanlaan 121
1066 CX, Amsterdam
Netherlands
