Spectrometers for Solid state NMR: 1.2 GHz, 900 MHz, 850MHz, 600 MHz, 400 MHz
For structural investigations of proteins and protein complexes using solid-state NMR spectroscopy, isotopically labeled samples are generally required. Typically, samples should be enriched with 15N and 13C isotopes. These labeled biomolecules must be prepared in advance by the visiting scientist. In addition, the samples have to be prepared at very high concentrations due to the limited volume available in the MAS NMR rotors. Typically, the protein will be prepared as a proteoliposom or precipitated in a suitable way. The Facility provides expert advice on optimal isotope-labeling strategies, labeling schemes, and sample preparation protocols suitable for the intended NMR experiments.
Facility support commonly includes instrument setup, data acquisition, and data analysis, depending on the user’s level of expertise and project requirements. Additionally, training sessions can be arranged to help visiting scientists gain proficiency in NMR data collection and interpretation.
Access to the Facility can be obtained either through a physical visit or via sample mail-in for remote measurements.
Researchers are strongly encouraged to contact the Facility staff, Johanna Becker-Baldus, prior to submitting a proposal, in order to discuss project details and ensure that experimental plans align optimally with available services and instrumentation.
BMRZ, Goethe-University Frankfurt (GUF)
