Journal of Visualized Experiments
Several standard and non-routine iNEXT-Discovery methods and protocols that are used by our facilities have been collected in a single Methods-issue of the Journal of Visualized experiments. These protocols appear as peer-reviewed open access articles, but are importantly also supported by video-footage prepared together with the facility staff. New contributions are included as the papers become public and filming proceeds.
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EDITORIAL |
Wienk H, Banci L, Daenke S, Pereiro E, Schwalbe H, Stuart DI, Weiss MS, Perrakis A. J Vis Exp. 2021; Nov 16:177 / doi: 10.3791/63435 |
iNEXT-Discovery and Instruct-ERIC: Integrating High-End Services for Translational Research in Structural Biology |  |
| FACILITY | WHO? | READ THE ARTICLE | WATCH THE PROTOCOLS |
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ABSL, Leeds (UK) cryo-EM
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White JB, Maskell DP, Howe A, Harrow M, Clare DK, Siebert CA, Hesketh EL, Thompson RF. J Vis Exp.; 2021, Mar 19:169 / doi: 10.3791/62415 |
Single Particle Cryo-Electron Microscopy: From Sample to Structure |
TO THE VIDEO! |
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ALBA, Barcelona (ES) X-ray Tomography
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Groen J, Sorrentino A, Aballe L, Oliete R, Valcarcel R, Okolo C, Kounatidis I Harkiolaki M, Perez-Berna AJ, Pereiro E. J Vis Exp.; 2021, Mar 15:169 / doi: 10.3791/62190 |
A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography |
TO THE VIDEO! |
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CEITEC, Brno (CZ) Cryo-EM FIB-milling |
Moravcová J, Pinkas M, Holbová R, Nováček J. J Vis Exp.; 2021, Nov 20:177 / doi: 10.3791/16235 |
Preparation and Cryo-FIB micromachining ofSaccharomyces cerevisiae for Cryo-Electron Tomography | TO THE VIDEO! |
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CIRMMP, Florence (IT) Real-time in-cell NMR |
Barbieri L, Luchinat E. J Vis Exp. 2021; Mar 9:169 / doi: 10.3791/62323 |
Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor | TO THE VIDEO! |
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CSIC, Madrid (ES) Cryo-EM analysis |
Jiménez-Moreno A, del Caño L, Martínez M, Ramírez-Aportela E, Cuervo A, Melero R, Sánchez-García R, Strelak D, Fernández-Giménez E, de Isidro-Gómez F, Herreros D, Conesa P, Fonseca Y, Maluenda D, Jiménez de la Morena J, Macías J, Losana P, Marabini R, Carazo J, Sorzano C. J Vis Exp.; 2021, May 29:171 / doi: 10.3791/62261 |
Cryo-EM and Single-Particle Analysis with Scipion |
TO THE VIDEO! |
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DIAMOND, Didcot (UK) Cryo-SIM |
Vyas N, Perry N, Okolo CA, Kounatidis I, Fish TM, Nahas KL, Jadhav A, Koronfel MA, Groen J, Pereiro E, Dobbie IM, Harkiolaki M. J Vis Exp.; 2021, May 28:171 / doi: 10.3791/62274 |
Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells |
TO THE VIDEO! |
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DIAMOND, Didcot (UK) X-ray screening |
Douangamath A, Powell A, Fearon D, Collins PM, Talon R, Krojer T, Skyner R, Brandao-Neto J, Dunnett L, Dias A, Aimon A, Pearce NM, Wild C, Gorrie-Stone T, von Delft F. J Vis Exp.; 2021, May 29:171 / doi: 10.3791/62414 |
Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source |
TO THE VIDEO! |
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DIAMOND, Didcot (UK) Microcrystal mounting |
Crawshaw AD, Beale EV, Warren AJ, Stallwood A, Duller G, Trincao J, Evans G. J Vis Exp.; 2021, Jun 17:172 / doi: 10.3791/62306 |
A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline |
TO THE VIDEO! |
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DIAMOND, Didcot (UK) Serial X-ray |
Horrell S, Axford D, Devenish NE, J Vis Exp.; 2021, Feb 26:168 / doi: 10.3791/62200 |
Fixed Target Serial Data Collection at Diamond Light Source |
TO THE VIDEO! |
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EMBL-GR, Grenoble (FR) X-ray automation |
Cornaciu I, Bourgeas R, Hoffmann G, Dupeux F, Humm AS, Mariaule V, Pica A, Clavel D, Seroul G, Murphy P, Márquez JA. J Vis Exp.; 2021, Jun 5:172 / doi: 10.3791/62491 |
The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble |
TO THE VIDEO! |
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EMBL-HD, Heidelberg (DE) Cryo-ET |
Weis F, Hagen WJH, Schorb M, Mattei S. J Vis Exp.; 2021, Mar 19:169 / doi: 10.3791/62383 |
Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition |
TO THE VIDEO! |
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GUF/BMRZ, Frankfurt am Main (DE) NMR screening |
Berg H, Wirtz Martin MA, Niesteruk A, Richter C, Sreeramulu S, Schwalbe H. J Vis Exp.; 2021, Jun 4:172 / doi: 10.3791/62262 |
NMR-based Fragment Screening in a Minimum Sample but Maximum Automation Mode |
TO THE VIDEO! |
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HZB, Berlin (DE) X-ray screening |
Wollenhaupt J, Barthel T, Lima GMA, Metz A, Wallacher D, Jagudin E, Huschmann FU, Hauß T, Feiler CG, Gerlach M, Hellmig M, Förster R, Steffien M, Heine A, Klebe G, Mueller U, Weiss, MS. J Vis Exp.; 2021, Mar 3:169 / doi: 10.3791/62208 |
Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin |
TO THE VIDEO! |
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NKI, Amsterdam (NL) nano-DSF screening |
Ahmad MUD, Fish, A, Molenaar J, Sreeramulu S, Richter C, Altincekic N, Schwalbe H, Wienk H, Perrakis A. J Vis Exp.; 2021, May 16:171 / doi: 10.3791/62469 |
Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery |
TO THE VIDEO! |
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UU, Utrecht (NL) Membrane ssNMR |
Beriashvili D, Schellevis RD, Napoli F, Weingarth M, Baldus M. J Vis Exp.; 2021, May 16:169 / doi: 10.3791/62197 |
High-Resolution Studies of Proteins in Natural Membranes by Solid-State NMR |
TO THE VIDEO! |